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6 diamidino 2 phenylindole  (Beyotime)


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    Structured Review

    Beyotime 6 diamidino 2 phenylindole
    6 Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+dapi/Beyo3D+DAPI+Staining+Solution/pm42908353-49-46-47
    Average 99 stars, based on 1 article reviews
    6 diamidino 2 phenylindole - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Sex-Stratified Characterization of Behavioral, Hippocampal, and Microglial Androgen Responses to Adolescent High-Fat Diet Consumption in Mice.
    Article Snippet: .. Finally, the cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; C1027, 1:600; Beyotime, Shanghai, China). ..

    Article Title: A Self-Regenerating Mixed-Valence FeO x -TiO 2 Nanoplatform for Mitochondrion-Targeted Triple-Modal Cancer Therapy
    Article Snippet: Anhydrous ethanol, hydrogen peroxide (30%), and dimethyl sulfoxide (DMSO) were acquired from Sinopharm Chemical Reagent Co., Ltd. (China). .. For biological assays, the MTT cell proliferation kit, Acridine Orange (AO) Staining Kit, Lipid Peroxidation (LPO) Assay Kit, MitoTracker Red CMXRos, and DAPI were sourced from Beyotime Biotechnology (China). .. The Mitochondrial Membrane Potential Assay Kit (JC-1) was from KeyGEN BioTECH Co., Ltd. (China).

    Article Title: SUGP1 Deficiency Leads to Splicing Dysregulation and Reduced piRNA Abundance in Insects
    Article Snippet: .. Fluorescent secondary antibodies (Cy3-conjugated goat anti-mouse IgG (H + L) and FITC-conjugated goat anti-rabbit IgG (H + L); Beyotime, Shanghai, China) were added and incubated in the dark at 37 ◦C for 1 h. Nuclei were stained with DAPI (1 μg/mL; Beyotime, Shanghai, China), and images were acquired using a confocal microscope. .. Fluorescence images were acquired using a Zeiss LSM 880 confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a 40×/1.4 NA oil-immersion objective.

    Article Title: Targeting V-ATPase assembly via sEVs-delivered doxorubicin triggers methuosis and circumvents temozolomide resistance in glioblastoma.
    Article Snippet: Aim: Temozolomide (TMZ) resistance remains a major challenge in glioblastoma (GBM).. Whether lysosomal alterations represent a targetable vulnerability in TMZ-resistant GBM (TR-GBM) is unclear.. This study investigates whether small extracellular vesicles (sEVs) can reprogram doxorubicin (DOX) subcellular trafficking to exploit lysosomal vulnerabilities and circumvent resistance.

    Article Title: Antagonism of PRDX1 and Japanese encephalitis virus via the autophagy pathway.
    Article Snippet: Then, 0.1% Triton X-100 (9284, Sigma-Aldrich) permeabilizing solution was added, followed by rinsing with PBS. .. Next, the cells were incubated for 1 h with the indicated primary antibodies and fluorescent conjugated secondary antibodies at 37°C, followed by 5 min of DAPI (C1002, Beyotime Biotechnology) staining. .. Finally, the samples were observed under a confocal immuno fluorescence microscope (Carl Zeiss, Oberkochen, Germany).

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: ADAR1 Inhibits ZBP1 ‐Driven Neuronal Necroptosis After Intracerebral Hemorrhage
    Article Snippet: Necroptosis is a critical response to secondary brain injury following intracerebral hemorrhage (ICH).. ZDNA Binding Protein 1 (ZBP1) binds to dsRNA and promotes the activation of ReceptorInteracting Protein Kinase 3 (RIPK3).. This study investigated whether Adenosine Deaminase Acting on RNA 1 (ADAR1) inhibits neuronal necroptosis after ICH.

    MTT Assay:

    Article Title: A Self-Regenerating Mixed-Valence FeO x -TiO 2 Nanoplatform for Mitochondrion-Targeted Triple-Modal Cancer Therapy
    Article Snippet: Anhydrous ethanol, hydrogen peroxide (30%), and dimethyl sulfoxide (DMSO) were acquired from Sinopharm Chemical Reagent Co., Ltd. (China). .. For biological assays, the MTT cell proliferation kit, Acridine Orange (AO) Staining Kit, Lipid Peroxidation (LPO) Assay Kit, MitoTracker Red CMXRos, and DAPI were sourced from Beyotime Biotechnology (China). .. The Mitochondrial Membrane Potential Assay Kit (JC-1) was from KeyGEN BioTECH Co., Ltd. (China).

    Incubation:

    Article Title: SUGP1 Deficiency Leads to Splicing Dysregulation and Reduced piRNA Abundance in Insects
    Article Snippet: .. Fluorescent secondary antibodies (Cy3-conjugated goat anti-mouse IgG (H + L) and FITC-conjugated goat anti-rabbit IgG (H + L); Beyotime, Shanghai, China) were added and incubated in the dark at 37 ◦C for 1 h. Nuclei were stained with DAPI (1 μg/mL; Beyotime, Shanghai, China), and images were acquired using a confocal microscope. .. Fluorescence images were acquired using a Zeiss LSM 880 confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a 40×/1.4 NA oil-immersion objective.

    Article Title: Antagonism of PRDX1 and Japanese encephalitis virus via the autophagy pathway.
    Article Snippet: Then, 0.1% Triton X-100 (9284, Sigma-Aldrich) permeabilizing solution was added, followed by rinsing with PBS. .. Next, the cells were incubated for 1 h with the indicated primary antibodies and fluorescent conjugated secondary antibodies at 37°C, followed by 5 min of DAPI (C1002, Beyotime Biotechnology) staining. .. Finally, the samples were observed under a confocal immuno fluorescence microscope (Carl Zeiss, Oberkochen, Germany).

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Confocal:

    Article Title: SUGP1 Deficiency Leads to Splicing Dysregulation and Reduced piRNA Abundance in Insects
    Article Snippet: .. Fluorescent secondary antibodies (Cy3-conjugated goat anti-mouse IgG (H + L) and FITC-conjugated goat anti-rabbit IgG (H + L); Beyotime, Shanghai, China) were added and incubated in the dark at 37 ◦C for 1 h. Nuclei were stained with DAPI (1 μg/mL; Beyotime, Shanghai, China), and images were acquired using a confocal microscope. .. Fluorescence images were acquired using a Zeiss LSM 880 confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a 40×/1.4 NA oil-immersion objective.

    Microscopy:

    Article Title: SUGP1 Deficiency Leads to Splicing Dysregulation and Reduced piRNA Abundance in Insects
    Article Snippet: .. Fluorescent secondary antibodies (Cy3-conjugated goat anti-mouse IgG (H + L) and FITC-conjugated goat anti-rabbit IgG (H + L); Beyotime, Shanghai, China) were added and incubated in the dark at 37 ◦C for 1 h. Nuclei were stained with DAPI (1 μg/mL; Beyotime, Shanghai, China), and images were acquired using a confocal microscope. .. Fluorescence images were acquired using a Zeiss LSM 880 confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a 40×/1.4 NA oil-immersion objective.

    Immunofluorescence:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Labeling:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Blocking Assay:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing